Fig 1: Knockdown of PLK4 inhibits tumor growth in vivo. (A) Representative images of isolated tumors in the control group (first row), shPLK4-1 group (second row) and shPLK4-2 group (third row). (B) Xenograft tumor volume was smaller in the shPLK4 groups compared with in the control group. (C) Xenograft tumor weight was reduced in the shPLK4 groups compared with in the control group. (D) Ki67-positive cells in subcutaneous xenograft tumor tissues developed from CRC cells. (E and F) Relative protein expression levels of pGSK3β, β-catenin, c-Myc, cyclin D1, p21 and epithelial-mesenchymal transition markers were examined in CRC xenograft tissues. (G) Relative protein expression levels of PLK4, β-catenin, pGSK3β, c-Myc, cyclin D1, p21, MMP1, MMP2, E-cadherin, N-cadherin, occludin and snail were semi-quantified. Results are presented as the means ± standard error of the mean of triplicate repeats from three independent experiments. *P<0.05, **P<0.01, ***P<0.001. CRC, colorectal cancer; GSK3β, glycogen synthase kinase 3β; MMP, matrix metallopro-teinase; p, phosphorylated; PLK4, polo-like kinase 4; sh/shRNA, short hairpin RNA; VEC, empty vector.
Fig 2: Immunohistochemical analysis of MMP1 (a) and computed tomography (CT) scanning images of a patient with IPF (b). DAB staining (brown color) indicates positive MMP1 expression in adjacent normal lung tissue of NSCLC without IPF or healthy normal tissue (a1-a3), IPF tissue (b1-b3), lung tumor tissue of NSCLC without IPF (c1-c3), IPF tissue of NSCLC with IPF (d), and tumor tissue of NSCLC with IPF (e). The IPF sample (b1-b3) is more severe than the IPF-progressed NSCLC sample from a patient who had not been diagnosed with lung cancer (d) and is also more severe than the IPF-progressed NSCLC sample from a patient who was diagnosed with lung cancer (a1-a2)
Fig 3: Effect of miR-27b on MMP1, α-SMA, collagen I and collagen III expression in BJ cells. MMP1, α-SMA, collagen I and collagen III expression in BJ cells was measured by (A) reverse transcription-quantitative PCR (B) western blot analysis. *P<0.05 vs. control; ^P<0.05 vs. MC; &P<0.05 vs. IC. miR, microRNA; MMP1, matrix metalloproteinase-1; α-SMA, α-smooth muscle actin; MC, miR-27b mimics control; IC, miR-27b inhibitor control. Data are expressed as the mean ± standard deviation of three experiments.
Fig 4: MMP1, α-SMA, collagen I and collagen III expression in rat skin tissues was measured using (A) reverse transcription-quantitative PCR and (B) western blot analysis. *P<0.05 vs. control group. #P<0.05 vs. model; ^P<0.05 vs. MC; &P<0.05 vs. IC. MMP1, matrix metalloproteinase-1; αSMA, α-smooth muscle actin; MC, miR-27b mimics control; miR, microRNA; IC, miR-27b inhibitor control. Data are expressed as the mean ± standard deviation of three experiments.
Fig 5: MMP1 knockdown inhibits cell proliferation and migration and invasion and triggers apoptosis in HNSCC cells. (a) Endogenous MMP1 protein expression was measured in a panel of HNSCC cell lines as compared to normal oral epithelial (HOK). Representative images of western blot (WB) were shown from 3 independent experiments. (b) Endogenous MMP1 was efficiently silenced by 2 siRNAs (siMMP1-1, siMMP1-2) in Cal27 and Fadu cells. Non-targeting siRNA was utilized as negative control (siNC). Representative images of WB are shown from 3 independent experiments. (c, d) Cell proliferation was remarkably suppressed when endogenous MMP1 was silenced as measured by CCK-8 viability assay. (e) The potentials of colony formation were significantly inhibited in MMP1-depleted cells as compared to control (siNC). (f, g) Increased percentages of cell undergoing apoptosis were evident following MMP1 knockdown as assayed by Annexin V-PI staining. (h, i) The migration (G) and invasion (H) abilities were significantly reduced in siMMP1-transfected cells in wound healing and transwell assays, respectively. (k–m) The protein and mRNA abundance of migration/invasion relevant marker E-cadherin, N-cadherin, Vimentin, and Snail was compared in cells infected siMMP1 or control siNC. (n) Correlation between generic EMT score for HNSCC samples from TCGA dataset and MMP1 expression. Generic EMT score was calculated following the method of single-sample Gene Set Enrichment Analysis (ssGSEA) [24]. Scale bar: 100 μm, Data shown here are mean ± SD from three independent experiments, ∗P < 0.05, ∗∗P < 0.01, ANOVA analyses.
Supplier Page from Abcam for Anti-MMP1 antibody